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Transcranial direct-current stimulation protects against cerebral ischemia-reperfusion injury through regulating Cezanne-dependent signaling.
Cheng, Jing; Fan, Yan-Qin; Jiang, Hong-Xiang; Chen, Song-Feng; Chen, Juan; Liao, Xin-Yu; Zou, Ying-Ying; Lan, Hai-Yang; Cui, Yu; Chen, Zhi-Biao; Chen, Qian-Xue; Wan, Qi.
Affiliation
  • Cheng J; Department of Neurosurgery, Renmin Hospital of Wuhan University,99 Zhang Zhidong Street, Wuhan 430060, China.
  • Fan YQ; Department of Nephrology, Renmin Hospital of Wuhan University,99 Zhang Zhidong Street, Wuhan 430060, China.
  • Jiang HX; Department of Neurosurgery, Renmin Hospital of Wuhan University,99 Zhang Zhidong Street, Wuhan 430060, China.
  • Chen SF; Department of Physiology, School of Medicine, Wuhan University, 185 Donghu Street, Wuhan 430071, China.
  • Chen J; Department of Neurology, the Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science & Technology, 26 Shengli Street, Wuhan 430013, China.
  • Liao XY; Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China.
  • Zou YY; Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, China.
  • Lan HY; Department of Orthopaedics, Army Medical Center (Daping Hospital), Army Medical University, Chongqing 400042, China.
  • Cui Y; Institute of Neuroregeneration & Neurorehabilitation, Department of Pathophysiology, Qingdao University, 308 Ningxia Street, Qingdao 266071, China.
  • Chen ZB; Department of Neurosurgery, Renmin Hospital of Wuhan University,99 Zhang Zhidong Street, Wuhan 430060, China.
  • Chen QX; Department of Neurosurgery, Renmin Hospital of Wuhan University,99 Zhang Zhidong Street, Wuhan 430060, China. Electronic address: chenqx666@whu.edu.cn.
  • Wan Q; Institute of Neuroregeneration & Neurorehabilitation, Department of Pathophysiology, Qingdao University, 308 Ningxia Street, Qingdao 266071, China. Electronic address: qiwan1@hotmail.com.
Exp Neurol ; 345: 113818, 2021 11.
Article in En | MEDLINE | ID: mdl-34324860
Transcranial direct-current stimulation (tDCS) is proved safe and shows therapeutic effect in cerebral ischemic stroke in clinical trials. But the underlying molecular mechanisms remain unclear. Here we show that tDCS treatment reduces the infarct volume after rat cerebral ischemia-reperfusion (I/R) injury and results in functional improvement of stroke animals. At the cellular and molecular level, tDCS suppresses I/R-induced upregulation of Cezanne in the ischemic neurons. Cezanne inhibition confers neuroprotection after rat I/R and oxygen glucose deprivation (OGD) in the cortical neuronal cultures. Inhibiting Cezanne increases the level of SIRT6 that is downregulated in the ischemic neurons. Suppressing SIRT6 blocks Cezanne inhibition-induced neuroprotective effect and overexpressing SIRT6 attenuates OGD-induced neuronal death. We further show that downregulating Cezanne reduces DNA double-strand break (DSB) through upregulation of SIRT6 in OGD-insulted neurons. Together, this study suggests that Cezanne-dependent SIRT6-DNA DSB signaling pathway may mediate the neuroprotective effect of tDCS in ischemic neurons.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endopeptidases / Reperfusion Injury / Signal Transduction / Brain Ischemia / Transcranial Direct Current Stimulation / Neuroprotection Limits: Animals / Pregnancy Language: En Journal: Exp Neurol Year: 2021 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endopeptidases / Reperfusion Injury / Signal Transduction / Brain Ischemia / Transcranial Direct Current Stimulation / Neuroprotection Limits: Animals / Pregnancy Language: En Journal: Exp Neurol Year: 2021 Document type: Article Affiliation country: Country of publication: